Patent · US Active

Method for generation of a cell composition of mesencephalic dopaminergic progenitor cells

US10072245B2 · kind B2 · utility

2Cited by

0References

7Claims

0Family size

Assignee

Miltenyi Biotec, Inc. · DE

Inventors

Andreas Bosio · Cologne, DE
Sebastian Knobel · Cologne, DE
Daniela Lehnen · Cologne, DE
Serena Barral · London, GB

Key dates

Filing date	Feb 26, 2016
Grant date	Sep 11, 2018
Priority date	—
Expiry date	Mar 2, 2036

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC12N2533/52
WIPO fieldPharmaceuticals
WIPO sectorChemistry

Abstract

The present invention provides a method for generation of a cell composition of mesencephalic dopaminergic progenitor cells from a starting cell composition comprising pluripotent and/or multipotent stem cells, the method comprising the steps of a) differentiating said pluripotent and/or multipotent stem cells into mesencephalic dopaminergic progenitor cells, thereby generating a cell population comprising mesencephalic dopaminergic progenitor cells and other cells, b) dissociating the differentiated cells of step a) into a single cell suspension, and c) enriching said mesencephalic dopaminergic progenitor cells by using an antigen binding molecule specific for the CD47 antigen for positive selection of said mesencephalic dopaminergic progenitor cells in said single cell suspension. Said method may be performed in a closed cell sample processing system and may be performed in an automated manner.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.