Patent · US Active

Method and device for preparing a sample of biological material containing target cells and accompanying cells for extracting nucleic acids of the target cells

US10072258B2 · kind B2 · utility

2Cited by

2References

7Claims

0Family size

Assignee

Robert Bosch GmbH · DE

Inventors

Bernd Faltin · Glückstadt, DE
Franz Laermer · Stuttgart, DE
Jochen Rupp · Stuttgart, DE
Peter Rothacher · Bruchsal, DE
Christian Dorrer · Winnenden, DE
Sebastian Berning · Stuttgart, DE

Key dates

Filing date	Jul 30, 2014
Grant date	Sep 11, 2018
Priority date	—
Expiry date	Jul 30, 2034

Classification

Technology area (CPC G)Physics
CPC primaryG01N29/00
WIPO fieldMeasurement
WIPO sectorInstruments

Abstract

A method for preparing a sample of biological materials containing target cells and accompanying cells for extracting nucleic acids of the target cells includes accumulating the target cells of the sample by separating the target cells or the accompanying cells from the sample. The method also includes digesting the target cells via chemical and/or physical lysis in order to produce a target cell lysate containing the nucleic acids of the target cells. The method furthermore includes purifying the nucleic acids from the target cell lysate in order to extract the nucleic acids of the target cells.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.