Microfluidic device and method for detecting rare cells

US10073024B2 · kind B2 · utility

Assignee

• THE REGENTS OF THE UNIVERSITY OF MICHIGAN · US

Inventors

• Sunitha Nagrath · Ann Arbor, US
• Hyeun Joong Yoon · Brookings, US
• Eric Lin · Baoshan, TW
• Max S. Wicha · Ann Arbor, US
• Lianette Rivera Baez · Ann Arbor, US
• Diane M. Simeone · Ann Arbor, US

Key dates

• Filing date: Oct 29, 2013
• Grant date: Sep 11, 2018
• Expiry date: Mar 13, 2034

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N2015/1028
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

A microfluidic device for detecting rare cells in a fluid sample comprises the rare cell and other cells. The microfluidic device comprises an inlet for receiving the fluid sample, a labyrinth channel structure in fluid communication with the inlet, and an outlet in fluid communication with the labyrinth channel structure for collecting the rare cells separated from the other cells in the fluid sample. The labyrinth channel structure comprises at least one channel through which the fluid sample flows. The at least one channel has a plurality of segments and a plurality of corners with each corner defined between adjacent segments. The presence of the plurality of corners induces separation of the rare cells from the other cells in the fluid sample as the rare cells move to a first equilibrium position within the at least one channel when a ratio of inertial lift forces (FZ) and Dean flow (FD) of the fluid sample is from 2 to 10.