Non-fucosylated protein and methods thereof

US10077434B2 · kind B2 · utility

Assignee

• Zumutor Biologics Inc. · US

Inventors

• Sohang Chatterjee · Kanchinakote, IN
• Kavitha Iyer Rodrigues · Bengaluru, IN
• Maloy Ghosh · Kanchinakote, IN
• Sunit Maity · Kanchinakote, IN
• Rajeshwari Pendse · Bengaluru, IN
• Divya Unnikrishnan · Kanchinakote, IN
• Yogendra Manjunath B. M. · Bengaluru, IN
• Jahnabi Arika · Bengaluru, IN
• Sathyabalan M. · Bengaluru, IN
• Pavithra M. · Kundapura, IN
• Bhargav Prasad · Komangalam, IN
• Veeresha K. · Bengaluru, IN
• Prabhat Kumar Pathak · Varanasi, IN
• Sanghamitra Bhattacharjee · Kanchinakote, IN
• Pravin Kumar D. · Chennai, IN
• Vivek Halan · Viralimalai, IN
• Sankaranarayanan Srinivasan · Kanchinakote, IN
• Anuradha Hora · Sitapur, IN
• Bairavabalakumar N. · Chennai, IN
• Karthika Nair · Survanagiri, IN
• Aswini Thanigaivel · Chennai, IN
• Amol Maliwalave · Survanagiri, IN
• Bharath Ravindra Shenoy · Kanchinakote, IN
• Anisha Kurup · Delhi, IN

Key dates

• Filing date: Jul 30, 2015
• Grant date: Sep 18, 2018
• Expiry date: Jul 30, 2035

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C07K2319/80
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The present disclosure relates to developing cell lines where specific biological pathways are modified, particularly by modifications in the enzymes of the cell. The present disclosure develops protein expression systems wherein specific modification of glycan chain of the protein is achieved, which produces non-fucosylated proteins, including non-fucosylated antibodies. The non-fucosylated proteins are used in developing therapeutic monoclonal antibodies and biomarkers, and in diagnosis and prognosis of various diseases. The present disclosure employs the Transcription Activator like Effector Nuclease (TALEN) technology for inactivating fucosylation in a cell.