Nucleic acid sequencing methods and systems
US10077470B2 · kind B2 · utility
16Cited by
35References
69Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Jul 21, 2015 |
| Grant date | Sep 18, 2018 |
| Priority date | — |
| Expiry date | Aug 21, 2036 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6869
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present disclosure provides compositions, methods and systems for sequencing a template nucleic acid using a polymerase based, nucleic acid binding reaction involving examination of the interaction between a polymerase and template nucleic acid in the presence of one or more unlabeled nucleotides. The methods rely, in part, on identifying a base of a template nucleic acid during nucleic acid synthesis by controlling the sequencing reaction conditions. Template nucleic acid bases may be identified during an examination step followed by an optional incorporation step.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.