Methods for sorting nucleic acids and multiplexed preparative in vitro cloning

US10081807B2 · kind B2 · utility

Assignee

• Gen9, Inc. · US

Inventors

• Joseph M. Jacobson · Newton, US
• Martin J. Goldberg · Saratoga, US
• Li-yun A. Kung · Arlington, US
• Daniel Schindler · Newton, US
• Michael E. Hudson · Portland, US

Key dates

• Filing date: Apr 24, 2013
• Grant date: Sep 25, 2018
• Expiry date: Apr 9, 2035

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N15/1093
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Methods and compositions relate to the sorting and cloning of high fidelity nucleic acids using high throughput sequencing. Specifically, nucleic acid molecules having the desired predetermined sequence can be sorted from a pool comprising a plurality of nucleic acids having correct and incorrect sequences.