Method and arrangement for calibrating a sensor element

US10081831B2 · kind B2 · utility

Assignee

• BOEHRINGER INGELHEIM VETMEDICA GMBH · DE

Inventors

• Katja Friedrich · Erlenbach a.Main, DE
• Walter Gumbrecht · Herzogenaurach, DE
• Peter Paulicka · Erlangen, DE
• Manfred Stanzel · Erlangen, DE
• Renee Weber · Langenau, DE

Key dates

• Filing date: Oct 29, 2008
• Grant date: Sep 25, 2018
• Expiry date: Sep 1, 2030

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q1/6837
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

A method is disclosed for calibrating a sensor element, which has an immobilized probe oligonucleotide, via which the bonding of a target nucleic acid (Z) can be detected by the sensor. In at least one embodiment, the method includes: a) bringing the sensor element into contact with a control nucleic acid (K), the melting temperature Tm(K) of which is less than the melting temperature Tm(Z) of the target nucleic acid (Z); b) hybridizing the control nucleic acid (K) to the probe oligonucleotide at a temperature T[p]<Tm(K), and detecting a positive control signal; and optionally c) modifying the stringent conditions such that T[n]>Tm(K) and detecting a negative control signal at a temperature T[n]. According to a refinement of at least one embodiment of the invention, a measuring signal of the target nucleic acid (Z) is measured at a measuring temperature T [mess], where Tm(K)<T[mess]<Tm(Z). The method is suited in particular for the calibration and quality control of microarrays.