miRNA profiling compositions and methods of use
US10086093B2 · kind B2 · utility
0Cited by
32References
18Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Feb 26, 2014 |
| Grant date | Oct 2, 2018 |
| Priority date | — |
| Expiry date | Nov 13, 2034 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q2600/178
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
Disclosed herein is a nanosensor of miRNA activity in a target cell, and methods of use, for detection and diagnostic applications. The nanosensor comprises a delivery particle comprising an iron oxide crystal coated with a polymer; and a sensor oligonucleotide covalently attached to the polymer. The sensor oligonucleotide comprises a seed region, comprising a nucleic acid sequence that is completely complementary to the target miRNA and comprises a cleavage site which can be engaged and cleaved by the target miRNA.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.