Culturing pluripotent stem cells
US10087416B2 · kind B2 · utility
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10Claims
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Key dates
| Filing date | Oct 27, 2014 |
| Grant date | Oct 2, 2018 |
| Priority date | — |
| Expiry date | Oct 27, 2034 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2533/90
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method for culturing and maintaining a pluripotent stem cell in an undifferentiated state is provided. The method comprises culturing the pluripotent stem cell in a medium comprising an MEK inhibitor, a GSK3 inhibitor, a dual inhibitor of AMPK and/or BMP signaling and LIF. A cell produced by the method, cell culture medium and a kit for performing the method described is also provided.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.