Patent · US Active

Amplification of bisulfite-reacted nucleic acids

US10087482B2 · kind B2 · utility

2Cited by

0References

8Claims

0Family size

Assignee

QIAGEN GmbH · DE

Inventors

Christian Korfhage · Hilden, DE
Dirk Loeffert · Haan, DE
Ralf Peist · Hilden, DE
Nicolas Rudinger · Goldkronach, DE

Key dates

Filing date	Feb 6, 2009
Grant date	Oct 2, 2018
Priority date	—
Expiry date	May 21, 2031

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC12Q1/6844
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

The present invention relates to a reaction mixture for the amplification of nucleic acids, the non-methylated cytosine bases of which have been converted to uracil bases by means of a bisulfition reaction. The invention also discloses methods for amplifying bisulfited nucleic acid and for determining the nucleic acid methylation state, and also kits based on the reaction mixture according to the invention.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.