Method for synthesizing gene using high-depth oligonucleotide tiling
US10087484B2 · kind B2 · utility
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Key dates
| Filing date | Jun 16, 2016 |
| Grant date | Oct 2, 2018 |
| Priority date | — |
| Expiry date | Feb 9, 2037 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6834
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Provided is a method for stably synthesizing an error-free gene using a high-depth oligonucleotide tiling, which includes designing an oligonucleotide fragment by an over-overlapping method, synthesizing the oligonucleotide fragment using DNA microarray, retrieving error-free oligonucleotide fragments retrieved by next generation sequencing, and assembling the error-free oligonucleotide fragments.
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