Methods, compositions and devices for amplification of nucleic acids

US10087491B2 · kind B2 · utility

Assignee

• BAYER CROPSCIENCE AKTIENGESELLSCHAFT · DE

Inventors

• Piet Soetaert · Laarne, BE
• Martin Leube · Gentbrugge, BE
• Bastian Budde
• Klaus Ochmann · Leverkusen, DE
• Michael Harnau · Leichlingen, DE

Key dates

• Filing date: Oct 18, 2013
• Grant date: Oct 2, 2018
• Expiry date: Nov 17, 2033

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q2600/16
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Methods, kits and compositions of matter are provided which allow amplification of nucleic acid or interest or DNA of interest, comprising the steps of isolating template nucleic acid or DNA from a biological sample using an alkaline extraction solution and directly adding the extract to a reaction mixture, under conditions to amplify the nucleic acids, wherein the isolated template nucleic acid or DNA in the alkaline extraction solution is not diluted or not neutralized prior to the addition to the reaction mixture.