Method for obtaining blood plasma from a whole blood sample

US10088469B2 · kind B2 · utility

Assignee

• QIAGEN GmbH · DE

Inventors

• Andy Wende · Hilden, DE
• Sabine Werner · Mülheim an der Ruhr, DE
• Ralf Himmelreich · Langenfeld, DE

Key dates

• Filing date: Aug 29, 2013
• Grant date: Oct 2, 2018
• Expiry date: Aug 29, 2033

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q1/6806
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

A method for obtaining blood plasma from a whole blood sample comprising the following steps a) contacting the whole blood sample with a composition (A) comprising at least one carboxylic acid, wherein the addition of the acidic composition (A) and optionally further additives to the whole blood sample provides a sample mixture having a pH that lies in a range from 2.5 to 5; b) binding red and white blood cells to a magnetic solid phase; wherein step a) and step b) can be performed sequentially or simultaneously, c) separating the solid phase with the bound cells from the remaining sample thereby providing blood plasma.