Methods for liquid direct fluorescent antibody intracellular virus detection
US10088481B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Jan 21, 2015 |
| Grant date | Oct 2, 2018 |
| Priority date | — |
| Expiry date | Jan 21, 2035 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2333/135
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The present invention describes a liquid direct fluorescence antibody assay that is rapid and sensitive to detect respiratory virus in infected cells. The assay includes centrifugation of the specimen, incubation of sample and reagents in solution, and detection of the absence or presence of respiratory virus. Sapogenin is used as a detergent to permeabilize the cells for entry of the monoclonal antibodies to react with intracellular antigens. The cells are stained with fluorescently labeled monoclonal antibodies against the viral antigens along with a background stain and a fluorescent nuclear stain. This counter staining decreases background and allows co-localization of antigen and nuclear structures for enhanced detection.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.