Patent · US Active

Subject anti-HCV antibody detection assays employing NS3 capture peptides

US10088483B2 · kind B2 · utility

2Cited by
19References
6Claims
0Family size

Assignee

Inventors

Key dates

Filing dateOct 28, 2015
Grant dateOct 2, 2018
Priority date—
Expiry dateNov 11, 2035

Classification

  • Technology area (CPC G)Physics
  • CPC primaryG01N2469/20
  • WIPO fieldMeasurement
  • WIPO sectorInstruments

Abstract

The present disclosure provides methods, kits, and compositions for detecting subject anti-HCV antibodies in a sample using NS3 capture peptides. In certain embodiments, at least two NS3 helicase (NS3h) capture peptides and at least two conjugate peptides (e.g., NS3h conjugate peptides) are employed together, which allows for a broad dynamic range of subject antibody detection in a one-step type assay. In other embodiments, methods are provided of detecting NS3-specific subject antibodies without the use of a reducing agent. In some embodiments, NS3-specific subject antibodies are detected with a ‘double shot’ of NS3 conjugate peptide (e.g., conjugate peptide added to a sample both before and after washing).

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.