Recombinant Bacillus subtilis for producing acetylglucosamine and construction method thereof
US10093936B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Dec 24, 2016 |
| Grant date | Oct 9, 2018 |
| Priority date | — |
| Expiry date | Dec 24, 2036 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2310/12
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention provides a recombinant Bacillus subtilis for producing acetylglucosamine and construction method thereof. The recombinant Bacillus subtilis is obtained by deletion of glmS ribozyme of Bacillus subtilis for regulating expression of glucosamine synthase, and insertion of a terminator and a constitutive promoter. The method comprises constructing a deleting cassette of a glmS ribozyme encoding gene, which includes an upstream homologous fragment, a resistance gene, a terminator sequence, a constitutive promoter sequence and a downstream homologous fragment in sequence; and transforming the deleting cassette into Bacillus subtilis, to obtain the recombinant Bacillus subtilis. In the invention, glucosamine synthase gene (glmS) ribozyme is deleted by homologous recombination, and in host cells, GlcN6P feedback inhibition of expression of glucosamine synthase gene glmS is blocked, and the accumulation of acetylglucosamine is improved.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.