Method for preserving cells and cell cultures
US10098340B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Feb 3, 2012 |
| Grant date | Oct 16, 2018 |
| Priority date | — |
| Expiry date | Jun 26, 2034 |
Classification
- Technology area (CPC A)Human Necessities
- CPC primaryA01N1/165
- WIPO fieldBasic materials chemistry
- WIPO sectorChemistry
Abstract
Provided is a method for reducing apoptosis in nucleated cells. The method entails holding nucleated cells in a container and adding a gas containing xenon to the container so that the pressure inside the container reaches between 0.5 to 4.0 Atm above ambient pressure; holding the container at between 0.5 to 4.0 Atm above ambient pressure for a period of time during which the temperature in the container is between 22° C. and 37° C.; lowering the temperature in the container to between 0.1° C. and 10° C. while maintaining the pressure of 0.5 to 4.0 Atm above ambient pressure and holding the container for a period of time; and reducing the pressure in the container to ambient pressure and increasing the temperature to 22° C.-37° C. By performing these steps, the cells undergo less apoptosis than a reference.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.