Split inteins and uses thereof

US10100080B2 · kind B2 · utility

Assignee

• ERA BIOTECH, S.A. · ES

Inventors

• Roser Pallisse Bergwerf · Sant Cugat del Vallès, ES
• Stefan Robert Schmidt · Laupheim, DE
• Didac Marco Feliu · Barcelona, ES
• Patricia Karina Carvajal Vallejos · Barcelona, ES

Key dates

• Filing date: Sep 28, 2012
• Grant date: Oct 16, 2018
• Expiry date: Mar 3, 2033

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C07K2319/92
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The present invention relates generally to robust split inteins. The split inteins described herein are active over a large temperature range, including temperatures as low as 0° C., over a wide pH range, and in the presence of chaotropic salts. The split inteins also show high tolerance to sequence variability in fused heterologous polypeptides and therefore are useful in protein purification and engineering techniques.