Use of a filter for defrosting cells
US10100276B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Jun 27, 2014 |
| Grant date | Oct 16, 2018 |
| Priority date | — |
| Expiry date | Sep 10, 2034 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2527/00
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The invention relates to a method for cell defrosting, comprising a step of defrosting a cell suspension consisting of a freezing medium and of cells, followed by a step of removing said freezing medium by filtration, said step of removing the freezing medium being carried out without the previous or simultaneous supply of medium. In particular, the filter is hydrophobic or, before addition of the cell suspension to be frozen, has been covered with a hydrophobic liquid forming a temporary barrier between the filter and the cell suspension. During defrosting, said hydrophobic liquid is removed by the application of a driving force which makes the liquid pass through the filter.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.