Production of squalene using yeast

US10100334B2 · kind B2 · utility

Assignee

• NUCELIS LLC · US

Inventors

• Keith A. M. Walker · Los Altos Hills, US
• Mark E. Knuth · Redwood City, US
• Noel M. Fong · San Diego, US
• Peter R. Beetham · Carlsbad, US

Key dates

• Filing date: May 22, 2009
• Grant date: Oct 16, 2018
• Expiry date: Jul 11, 2033

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12P5/007
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Provided herein compositions and methods for producing isoprenoids, including squalene. In certain aspects and embodiments provided are genetically altered yeast and uses therefore. In some aspects and embodiments, the genetically altered yeast produce isoprenoids, preferably squalene. The genetically altered yeast may have alterations in the expression or activity of enzymes involved in squalene production, for example, acetyl-CoA carboxylase (or “ACCase”), HMG-CoA reductase, squalene epoxidase, and squalene synthase. One or more genes of a genetically altered yeast may be modified by gene repair oligonucleobases. Also are provided methods of producing squalene using a genetically altered yeast. The invention also provides squalene produced by genetically altered yeast.