Methods and compositions for reducing redundant molecular barcodes created in primer extension reactions
US10100358B2 · kind B2 · utility
5Cited by
17References
21Claims
0Family size
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Key dates
| Filing date | Jan 10, 2018 |
| Grant date | Oct 16, 2018 |
| Priority date | — |
| Expiry date | Jan 10, 2038 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/686
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Methods, compositions, systems and kits to introduce a controlled-number of molecular barcodes onto DNA fragments by reducing redundant molecular barcodes formed in template-dependent primer extension reactions, and to find variant frequencies from both strands of DNA. The methods, compositions, systems and kits described herein may include, or include the use of, one or more single-stranded DNA specific nucleases that cleave the single-stranded regions containing unmatched base pairs in amplification products.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.