Compositions and methods for detecting huanglongbing
US10100370B2 · kind B2 · utility
6Cited by
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10Claims
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Key dates
| Filing date | Apr 28, 2015 |
| Grant date | Oct 16, 2018 |
| Priority date | — |
| Expiry date | Jun 16, 2035 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q2600/156
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention features compositions and methods for detecting Huanglongbing (HLB) in citrus trees and insects. In one aspect, the invention provides a method of detecting a Huanglongbing (HLB) infection in a citrus grove involving obtaining an extract from a biological sample derived from a citrus grove, contacting the extract with forward and reverse primers that specifically bind a Candidutus nucleic acid molecule in the presence of a nicking enzyme, dNTPs, and a polymerase under conditions permissive for the isothermal amplification of the nucleic acid molecule; and detecting a Candidutus amplicon in the extract.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.