Transgenic cells with increased plastoquinone levels and methods of use

US10106809B2 · kind B2 · utility

Assignee

• LOS ALAMOS NATIONAL SECURITY, LLC · US

Inventors

• Richard T. Sayre · Worthington, US
• Sowmya Subramanian · Los Alamos, US
• Edgar Cahoon · Wilmington, US

Key dates

• Filing date: Dec 22, 2016
• Grant date: Oct 23, 2018
• Expiry date: Dec 22, 2036

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y02A40/146
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Disclosed herein are transgenic cells expressing a heterologous nucleic acid encoding a prephenate dehydrogenase (PDH) protein, a heterologous nucleic acid encoding a homogentisate solanesyl transferase (HST) protein, a heterologous nucleic acid encoding a deoxyxylulose phosphate synthase (DXS) protein, or a combination of two or more thereof. In particular examples, the disclosed transgenic cells have increased plastoquinone levels. Also disclosed are methods of increasing cell growth rates or production of biomass by cultivating transgenic cells expressing a heterologous nucleic acid encoding a PDH protein, a heterologous nucleic acid encoding an HST protein, a heterologous nucleic acid encoding a DXS protein, or a combination of two or more thereof under conditions sufficient to produce cell growth or biomass.