Ribozyme with tRNA synthetase activity and methods of manufacturing and using the same
US10113168B2 · kind B2 · utility
Assignees
Inventors
Key dates
| Filing date | Jul 13, 2016 |
| Grant date | Oct 30, 2018 |
| Priority date | — |
| Expiry date | Jul 13, 2036 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2310/128
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Ribozymes exhibiting tRNA synthetase activity and substrate specificity, as well as methods for engineering and producing the same, are disclosed. The ribozymes of the present disclosure comprise a T-box RNA module fused with a flexizyme module. The flexizyme module provides high promiscuity with respect to amino acid substrates and the T-box module provides tRNA substrate specificity. Systems are also described for aminoacylation of suppressor tRNAs with unnatural amino acids (uAAs), such systems comprising the ribozyme previously mentioned, suppressor tRNA, and the desired uAAs. Methods for incorporating a uAA into a growing polypeptide chain using the ribozyme hereof are also provided.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.