Specific detection and quantification of cardiolipin and isolated mitochondria by positively charged AIE fluorogens and method of manufacturing thereof
US10113968B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Dec 3, 2014 |
| Grant date | Oct 30, 2018 |
| Priority date | — |
| Expiry date | Mar 8, 2035 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/483
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The present subject matter relates to a one-step method of detecting and quantifying cardiolipin in a sample using a positively charged AIE luminogen by introducing the AIE luminogen to a solution containing the sample and measuring fluorescence intensity of the solution; a method of quantifying isolated mitochondria using a positively charged AIE luminogen by staining a sample containing isolated mitochondria with the AIE luminogen and measuring the fluorescence intensity; and a method of quantifying isolated mitochondria using a positively charged AIE luminogen by introducing the AIE luminogen to a sample containing isolated mitochondria, wherein the AIE luminogen stains the isolated mitochondria and identifying the stained isolated mitochondria under microscope. With improved sensitivity and excellent selectivity to CL over other major mitochondrial membrane lipids, an aggregation-induced emission-active fluorogen, TTAPE-Me, may serve as a valuable fluorescent sensor for CL detection and quantification and the quantification of isolated mitochondria.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.