Compositions and methods for producing clostridial collagenases
US10119131B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Mar 16, 2012 |
| Grant date | Nov 6, 2018 |
| Priority date | — |
| Expiry date | Sep 9, 2032 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12P21/06
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
The present invention provides a method for producing a drug product comprising a combination of highly purified collagenase I and collagenase II from Clostridium histolyticum. The method utilizes an improved medium for the cultivation of Clostridium histolyticum which includes a non-meat-derived (i.e., non-mammalian) peptone or vegetable peptone. The method includes one or more of: (1) reducing glucose content in the meat-free or vegetable-derived media; and (2) increasing the salt concentration in the meat-free or vegetable-derived media. Also provided is a drug product which includes collagenase I and collagenase II at an optimized fixed mass ratio, and which has a purity of greater than at least 95%.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.