Using truncated guide RNAs (tru-gRNAs) to increase specificity for RNA-guided genome editing

US10119133B2 · kind B2 · utility

Assignee

• The General Hospital Corporation · US

Inventors

• J. Keith Joung · Winchester, US
• Jeffry D. Sander · Ankeny, US
• Yan Fu · Shanghai, CN
• Morgan Maeder · Brookline, US

Key dates

• Filing date: Mar 14, 2014
• Grant date: Nov 6, 2018
• Expiry date: Mar 14, 2034

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Y301/00
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

CRISPR-Cas genome editing uses a guide RNA, which includes both a complementarity region, which binds the target DNA by base-pairing, and a Cas9-binding region, to direct a Cas9 nuclease to a target DNA. Further disclosed are methods for increasing specificity of RNA-guided genome editing using CRISPR/Cas9 systems by using truncated guide RNAs (tru-gRNAs).