Method for secretion of extracellular vesicles from cells and tissues using shock wave
US10125360B2 · kind B2 · utility
Assignee
Inventor
Key dates
| Filing date | Jan 20, 2017 |
| Grant date | Nov 13, 2018 |
| Priority date | — |
| Expiry date | Jan 20, 2037 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2320/32
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention relates to a method for promoting the generation and secretion of extracellular vesicles from cells by using extracorporeal shockwave. More precisely, the inventors confirmed that the generation and secretion of extracellular vesicles from vascular endothelial cells were promoted by extracorporeal shockwave and further confirmed that siRNA can be transferred into cells without any help of a carrier. The inventors continued to find out that angiogenesis could be inhibited by introducing siRNA to an animal tumor model and succeeded to measure the size and amount of extracellular vesicles secreted by extracorporeal shockwave. Therefore, the present invention can be used in various fields since it can safely promote the generation and secretion of extracellular vesicles from cells.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.