Method for producing L-citrulline by using a recombinant Corynebacterium crenatum strain
US10125383B2 · kind B2 · utility
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Key dates
| Filing date | Jan 22, 2018 |
| Grant date | Nov 13, 2018 |
| Priority date | — |
| Expiry date | Jan 22, 2038 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12R2001/15
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Disclosed is a method for producing L-citrulline using recombinant Corynebacterium crenatum cells as whole-cell biocatalysts. The present invention provides a recombinant C. crenatum that expresses an exogenous arginine deiminase gene from Lactobacillus brevis. The recombinant C. crenatum SDNN403 is used as biocatalysts for converting L-arginine to produce L-citrulline. Using the method of the invention, the concentration of L-citrulline reached 301.4 g/L after a 48 hr conversion reaction, and the molar conversion rate reached 99.9%.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.