Reagent cartridge and methods for detection of cells

US10125386B2 · kind B2 · utility

Assignee

• GeneWeave Biosciences, Inc. · US

Inventors

• Nikol de Forest · Scotts Valley, US
• Werner Frei · Los Gatos, US
• Diego Ariel Rey · Palo Alto, US
• Shaunak Roy · Palo Alto, US
• Soni Shukla · Santa Clara, US
• Ryan Griswold · Los Gatos, US
• Kenneth G. Olson · San Jose, US
• Bruce J. Richardson · Los Gatos, US
• Victor H. Yee · Castro Valley, US

Key dates

• Filing date: Nov 30, 2016
• Grant date: Nov 13, 2018
• Expiry date: Jan 31, 2037

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N2021/7786
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

An apparatus includes a housing and an actuator. The housing, which defines a reagent volume that can receive a reagent container, can be removably coupled to a reaction chamber. The housing includes a puncturer that defines a transfer pathway in fluid communication with the reagent volume. A delivery portion of the housing defines a delivery pathway between the transfer pathway and the reaction chamber when the housing is coupled to the reaction chamber. The actuator has a plunger portion disposed within the reagent volume. An engagement portion of the actuator can be manipulated to move the plunger portion within the reagent volume to deform the reagent container. The puncturer can pierce a frangible portion of the reagent container to convey a reagent from the reagent container into the reaction chamber via the transfer pathway and/or the delivery pathway.