Pure spectrum extraction from biological samples in fluorescence multispectral imaging
US10126242B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Jul 9, 2015 |
| Grant date | Nov 13, 2018 |
| Priority date | — |
| Expiry date | Sep 1, 2036 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2201/12
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The disclosure features methods that include obtaining multispectral image information for a sample that includes a fluorescent dye, calculating from an image cube of the information a first spectrum and a second spectrum, and calculating a pure spectrum of the fluorescent dye in the sample based on the first and second spectra, where a relative contribution of light emission from the fluorescent dye to the second spectrum is larger than a relative contribution of light emission from the fluorescent dye to the first spectrum, where calculating the first and second spectra includes identifying corresponding first and second sets of pixel intensity values in the image cube, and where identifying the first set of pixel intensity values includes designating one or more layers of the image cube as a first layer set.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.