Candida antarctica lipase B mutant, and methods for making and using the same
US10131889B1 · kind B1 · utility
Assignee
Inventors
Key dates
| Filing date | Nov 11, 2017 |
| Grant date | Nov 20, 2018 |
| Priority date | — |
| Expiry date | Nov 11, 2037 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Y301/01003
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention relates to the field of bioengineering. It provides a Candida antarctica lipase B mutant and its application. The mutant enzyme overcomes the limit of the parent enzyme that can exhibit high enantioselectivity towards (R)-3-TBDMSO glutaric acid methyl monoester only at temperatures below 5° C. The mutant enzyme successfully increased R-ee value at 5-70° C. The mutant D223V/A281S exhibits high R-ee value (>99%), high conversion rate (80%), and high space-time yield (107.54 g L−1 d−1). The present invention lays a foundation for industrial production of (R)-3-TBDMSO glutaric acid methyl monoester using a biosynthesis approach and provide insights into conformational dynamics-based enzyme design.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.