Method for using heat-resistant mismatch endonuclease
US10131890B2 · kind B2 · utility
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Key dates
| Filing date | Mar 13, 2014 |
| Grant date | Nov 20, 2018 |
| Priority date | — |
| Expiry date | Jan 30, 2035 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Y301/21
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Provided are a mismatch-specific cleavage reaction using a novel heat-resistant mismatch nuclease, a method for removing errors in a nucleic acid amplification reaction using the mismatch nuclease, a method for inhibiting the amplification of a nucleic acid having a specific base sequence during a nucleic acid amplification reaction, and a method for detecting a nucleic acid having a single-base polymorphic mutation using this inhibition method.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.