Method for assaying a protease
US10131932B2 · kind B2 · utility
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Key dates
| Filing date | Mar 10, 2016 |
| Grant date | Nov 20, 2018 |
| Priority date | — |
| Expiry date | Jun 14, 2036 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2800/224
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A method of determining generation of an activated protease in a biological sample is provided. The method comprises the steps of exposing a biological sample to a substrate for the activated protease, wherein the substrate comprises a detectable label linked to a cleavage sequence for the activated protease by C-terminal and N-terminal spacers that form a beta-sheet, and wherein the detectable label emits a first signal associated with the substrate and second signal associated with a cleaved product; and determining the generation of the activated protease by measuring the change in the first or second signal over time.
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