Application of thiolated single-stranded DNA in polymerase chain reaction
US10131941B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Aug 21, 2014 |
| Grant date | Nov 20, 2018 |
| Priority date | — |
| Expiry date | Sep 26, 2035 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6848
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
An application of thiolated single-stranded DNA enhances specific amplification of polymerase chain reaction, namely a method for enhancing specific amplification of polymerase chain reaction by utilizing thiolated single-stranded DNA, which includes the following step: adding an appropriate amount of the thiolated single-stranded DNA into a PCR system to perform PCR amplification, wherein the appropriate amount means that the final concentration of the thiolated single-stranded DNA in a 20 μL reaction system is not less than 15 μM. The thiolated single-stranded DNA meets the following conditions: the thiolated single-stranded DNA is one segment of any sequence which is non-complementary and non-homologous to a target sequence; the Tm value is not less than 37.7° C.; and at least one end contains a thiolalkyl group SH—C6H12—.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.