Enhanced isotachophoresis assays using additives with spatial gradients

US10132775B2 · kind B2 · utility

Assignee

• The Board of Trustees of the Leland Stanford Junior University · US

Inventors

• Juan G. Santiago · Fremont, US
• Alexandre Persat · Princeton, US
• Giancarlo Garcia · Stanford, US
• Charbel Eid · Stanford, US

Key dates

• Filing date: Aug 17, 2015
• Grant date: Nov 20, 2018
• Expiry date: Jul 4, 2036

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N27/44795
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

Techniques for enhanced isotachophoresis assays using additives with spatial gradients include forming a concentration gradient of an additive along a channel from an input port to an output port. The channel is used for isotachophoresis with ions of a leading electrolyte having a first mobility greater than a mobility of an analyte, and ions of a trailing electrolyte having a second mobility less than the mobility of the analyte. The additive is different from both the leading electrolyte and the trailing electrolyte; and the additive has a third mobility that assures the analyte will encounter the additive. The method further comprises introducing a mixture of the trailing electrolyte and a sample including the analyte. The method further comprises applying an electric field to the channel; and, measuring the analyte.