Cell lines for virus production and methods of use

US10137188B2 · kind B2 · utility

Assignees

• University of Georgia Research Foundation, Inc. · US
• The United States of America as Represented by the Secretary, Dept. of Health and Human Services · US
• Thermo Fisher Scientific Inc. · FI

Inventors

• Jon Karpilow · Boulder, US
• Mark Steven Oberste · Lilburn, US
• Ralph A. Tripp · Watkinsville, US
• Stephen M. Tompkins · Watkinsville, US

Key dates

• Filing date: Feb 5, 2014
• Grant date: Nov 27, 2018
• Expiry date: Apr 3, 2034

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2770/32651
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

Provided herein are engineered cell lines. In some embodiments, cells of an engineered cell line have altered expression of a gene and/or altered expression of an miRNA, wherein the altered expression results in increased or decreased production of a virus. The virus is a picornavirus, such as a poliovirus or Enterovirus 71. Also provided herein are methods for using the engineered cells to produce virus, and methods for treating a subject having or at risk of having a viral infection.