Fluorogen activating and shifting tag (FAST)

US10138278B2 · kind B2 · utility

Assignees

• PARIS SCIENCES ET LETTRES - QUARTIER LATIN · FR
• CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE · FR
• Universite Pierre Et Marie Curie (Paris 6) · FR

Inventors

• Arnaud Gautier · Paris, FR
• Ludovic JULLIEN · Arcueil, FR

Key dates

• Filing date: Jul 3, 2015
• Grant date: Nov 27, 2018
• Expiry date: Jul 3, 2035

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C07K2319/60
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

Disclosed is a functional derivative of a Photoactive Yellow Protein (PYP), or a functional fragment thereof, for fluorescently labelling particles, e.g. proteins, or surfaces, which is capable of binding reversibly a fluorogenic chromophore of formula (I), and which is capable of enhancing the brightness of the fluorogenic chromophore upon complexation thereto; and of inducing the spectral shift of the fluorogenic chromophore through the ionization of an auxochromic group thereof.