Method for producing antibody using “naked” expression vector expressing type II transmembrane fusion protein

US10138289B2 · kind B2 · utility

Assignee

• Delphi Genetics S.A. · BE

Inventors

• Jean-Christophe Renauld · Bouillon, BE
• Muriel Lemaire · Ottignies, BE
• Laurie Dumoutier · Orbais, BE
• Yannick Nizet · Lincent, BE
• Alain Vanderplasschen · Tubize, BE
• Laurent Gillet · Tenneville, BE
• Sophie Scohy · Nivelles, BE
• Cedric Szpirer · Fleurus, BE

Key dates

• Filing date: Jan 5, 2016
• Grant date: Nov 27, 2018
• Expiry date: Jan 5, 2036

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2710/24134
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

Methods are disclosed for generating antibodies and an expression vector used to express protein(s) which provoke the antibody response. The expression vector may be useful in generating an antibody directed to an antigen, comprising a gene in operable linkage with a promoter, which gene encodes upon expressing a fusion protein comprising (i) CD134L, a fragment, homologous or orthologues protein thereof as N-terminal moiety of the fusion protein; and (ii) all or part of an antigenic protein as C-terminal moiety of the fusion protein. To generate the antibodies, the vector is injected into a subject animal, which produces a fusion protein, against which antibodies are generated.