Patent · US Active

Method of producing secretable antibodies by expression in saccharomyces cerevisiae

US10138477B2 · kind B2 · utility

0Cited by

2References

9Claims

0Family size

Assignee

Merck Patent GmbH · DE

Inventors

Ralph Guenther · Griesheim, DE
Bjoern Hock · Maintal, DE
Stefan Becker · Darmstadt, DE
Laura Rhiel · Frankfurt am Main, DE

Key dates

Filing date	Dec 11, 2013
Grant date	Nov 27, 2018
Priority date	—
Expiry date	Dec 11, 2033

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC07K2317/624
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

The invention relates to a method for the production and non-covalent surface display of antibodies and derived fragments as well as molecule libraries based thereon on the surface of S. cerevisiae cells. The non-covalent manner of the surface display renders possible the selection of specific variants by means of high throughput screening and the subsequent switchable secretion of the selected binding molecule into the culture supernatant for biochemical characterization.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.