Manufacturing methods for production of RNA transcripts

US10138507B2 · kind B2 · utility

Assignee

• ModernaTX, Inc. · US

Inventors

• Stephane Bancel · Cambridge, US
• William Issa · Dedham, US
• John Grant Aunins · Cambridge, US
• Tirtha Chakraborty · Medford, US

Key dates

• Filing date: Mar 13, 2014
• Grant date: Nov 27, 2018
• Expiry date: Mar 13, 2034

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q1/6865
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Described are methods for production of RNA transcripts using a non-amplified, linearized DNA template in an in vitro transcription reaction. Enzymatic 5′ capping and oligo dT purification can also be included in the methods.