Annealing control primer and its uses
US10138518B2 · kind B2 · utility
Assignee
Inventor
Key dates
| Filing date | Dec 3, 2013 |
| Grant date | Nov 27, 2018 |
| Priority date | — |
| Expiry date | Sep 16, 2034 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6853
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention relates to an annealing control primer for improving annealing specificity in nucleic acid amplification and its applications to all fields of nucleic acid amplification-involved technology. The present primer comprises (a) a 3′-end portion having a hybridizing nucleotide sequence substantially complementary to a site on a template nucleic acid to hybridize therewith; (b) a 5′-end portion having a pre-selected arbitrary nucleotide sequence; and (c) a regulator portion positioned between said 3′-end portion and said 5′-end portion comprising at least one universal base or non-discriminatory base analog, whereby said regulator portion is capable of regulating an annealing portion of said primer in association with annealing temperature.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.