Phospholipase C mutant and use thereof
US10144919B2 · kind B2 · utility
2Cited by
1References
5Claims
0Family size
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Key dates
| Filing date | Nov 4, 2014 |
| Grant date | Dec 4, 2018 |
| Priority date | — |
| Expiry date | Jan 10, 2035 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Y301/04003
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present application provides mutants of wild type phospholipase C (PLC) specific to phosphatidylcholine from Bacillus cereus. The related mutations include mutation of asparagine at position 63 to another amino acid, also including mutation of arginine at position 20 to histidine and of alanine at position 83 to aspartic acid. The present application also provides a nucleic acid molecule encoding the mutant, a vector containing the nucleic acid molecule, and a cell containing the nucleic acid molecule or vector. The present application also provides uses of the mutant, nucleic acid molecule vector, and cell.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.