Differential tagging of RNA for preparation of a cell-free DNA/RNA sequencing library

US10144962B2 · kind B2 · utility

Assignee

• GRAIL, INC. · US

Inventors

• Matthew H. Larson · San Francisco, US
• H. John Kim · San Francisco, US
• Nick Eattock · Hercules, US
• Arash Jamshidi · Menlo Park, US

Key dates

• Filing date: Jun 29, 2017
• Grant date: Dec 4, 2018
• Expiry date: Jun 29, 2037

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q1/6806
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

In various aspects, the present disclosure provides methods, compositions, reactions mixtures, kits, and systems for sequencing both RNA and DNA from a single source sample. In some embodiments, RNA is treated so as to differentiate RNA sequences from DNA sequences derived from the same sample. In some embodiments, the RNA and DNA are cell-free polynucleotides.