Accurate and interference-free multiplexed quantitative proteomics using mass spectrometry

US10145818B2 · kind B2 · utility

Assignee

• President and Fellows of Harvard College · US

Inventors

• Martin Helmut Wuhr · Malden, US
• Steven P. Gygi · Cambridge, US
• Wilhelm Haas · Cambridge, US
• Graeme C. McAlister · San Jose, US
• Leonid Peshkin · Boston, US
• Ramin Rad · Los Angeles, US
• Marc W. Kirschner · Newton, US

Key dates

• Filing date: Oct 22, 2013
• Grant date: Dec 4, 2018
• Expiry date: Oct 22, 2033

Classification

• Technology area (CPC H): Electricity
• CPC primary: H01J49/0072
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

Embodiments are directed to a method, a computer readable medium encoded with instructions that, when executed, perform a method, and a system for performing mass spectrometry analysis. Molecules of different samples may be labeled with a chemical tag, allowing a multiplexed analysis of multiple samples. The labeled molecules may be fragmented, each fragmented molecule creating at least two separate ions. The relative abundance of each of the heavier ions, which may comprise the original molecule from the sample, may be measured. A relative abundance of the labeled molecules in each of the samples may be determined from the measured relative abundances of the heavier ions.