Silicone surfactants for emulsion assays

US10150786B2 · kind B2 · utility

Assignee

• Bio-Rad Laboratories, Inc. · US

Inventors

• Erin R. Chia · Walnut Creek, US
• Amy L. Hiddessen · Tracy, US
• Benjamin Hindson · Pleasanton, US
• Adam Lowe · Tracy, US
• Chunxiao Han · Dublin, US

Key dates

• Filing date: Sep 6, 2017
• Grant date: Dec 11, 2018
• Expiry date: Sep 6, 2037

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C08G77/70
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

System, including methods and compositions, for making and using emulsions that include a silicone oil and a silicone surfactant. The emulsions may include aqueous droplets disposed in a continuous phase that includes a silicone oil and a silicone surfactant. The aqueous droplets may contain an analyte, optionally at partial occupancy, and/or a luminescent (e.g., photoluminescent) reporter. An assay of the analyte may be performed with the droplets. In some cases, signals may be detected from the droplets, and a characteristic of the analyte, such as an analyte level or activity, may be determined based on the signals.