Stabilized reverse transcriptase fusion proteins

US10150955B2 · kind B2 · utility

Assignee

• The Board of Regents of the University of Texas System · US

Inventors

• Alan M. Lambowitz · Austin, US
• Sabine Mohr · Austin, US
• Georg Mohr · Heringen (Werra), DE
• Eman Ghanem · Austin, US

Key dates

• Filing date: May 25, 2016
• Grant date: Dec 11, 2018
• Expiry date: May 25, 2036

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C07K2319/24
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Stabilized reverse transcriptase fusion proteins including a thermostable reverse transcriptase connected to a stabilizer protein are described. Attaching the stabilizer protein to the thermostable reverse transcriptase stabilizes the fusion protein and can aid in its purification, provide increased solubility, allow for longer storage, or allow the fusion protein to be used under more rigorous conditions such as higher temperature. The stabilized reverse transcriptase fusion protein can also include a linker between the stabilizer protein and the thermostable reverse temperature. The stabilized reverse transcriptase fusion proteins are suitable for use in nucleic acid amplification methods such as the reverse transcription polymerase chain reaction and other applications involving cDNA synthesis.