Two-color fluorescence localization super-resolution biological microscopy method and system
US10151697B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Sep 7, 2015 |
| Grant date | Dec 11, 2018 |
| Priority date | — |
| Expiry date | Sep 7, 2035 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2201/06113
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A two-color fluorescence localized super-resolution biological microscopy method and system are disclosed. The method includes: performing two-color fluorescence labeling on a biological sample by using Alexa647 and Alexa750 fluorescent molecules or Cy5 and Cy7 fluorescent molecules, and soaking the biological sample in an imaging buffer solution; illuminating the biological sample by laser to generate a flashing fluorescent signal of a first channel and a flashing fluorescent signal of a second channel respectively; constructing a super-resolution image of a first biological structure and a super-resolution image of a second biological structure respectively; and aligning the super-resolution image of the first biological structure and the super-resolution image of the second biological structure so as to construct a super-resolution image of a third biological structure. Thereby, the present method and system do not generate channel crosstalk and can significantly reduce background noise, so that the imaging quality is improved.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.