Cells prepared by transient transfection and methods of use thereof

US10155038B2 · kind B2 · utility

Assignee

• Yale University · US

Inventors

• Peter M. Rabinovich · Madison, US
• Sherman M. Weissman · New Haven, US
• Marina E. Komarovskaya · Milford, US
• Erkut Bahceci · Hamden, US
• Samuel Katz · Woodbridge, US
• Efim Golub · New Haven, US

Key dates

• Filing date: Jan 27, 2016
• Grant date: Dec 18, 2018
• Expiry date: Jan 27, 2036

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2830/008
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

Compositions and methods of making cells using RNA, and cells made using the disclosed compositions and methods are also provided. In exemplary embodiments, RNA is transfected into cells to effect a molecular, biological, physiological, or histological change in the cells. In preferred embodiments, the RNA is prepared in vitro, more preferably using a DNA template according to the provided compositions and methods. Methods for treating or inhibiting a disorder or disease such cancer are also provided. The methods can include, for example, locally or systemically administering to the host an effective amount of one or more RNAs; or an effective amount of population of cells isolated from the subject or a syngeneic or histocompatible subject, contacted ex vivo with one or RNAs, and optionally expanded. The cells can be, for example, immune cells or stem cells.