Methods for purification of messenger RNA
US10155785B2 · kind B2 · utility
13Cited by
248References
16Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Dec 4, 2017 |
| Grant date | Dec 18, 2018 |
| Priority date | — |
| Expiry date | Dec 4, 2037 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/1017
- WIPO fieldOrganic fine chemistry
- WIPO sectorChemistry
Abstract
The present invention provides, among other things, methods of purifying messenger RNA (mRNA) including the steps of (a) precipitating mRNA from an impure preparation; (b) subjecting the impure preparation comprising precipitated mRNA to a purification process involving membrane filtration such that the precipitated mRNA is captured by a membrane; and (c) eluting the captured precipitated mRNA from the membrane by re-solubilizing the mRNA, thereby resulting in a purified mRNA solution. In some embodiments, a purification process involving membrane filtration suitable for the present invention is tangential flow filtration.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.